Leafy vegetables moving through a washing line
Cleaning & Washing > Food washing

Effects of nanobubbles on food washing

Changes in microorganisms and residues when produce and food are washed with fine bubbles.

Key results

77.3%

Pesticide removal

Apple. Air micro-nanobubbles combined with an antimicrobial (Lu et al., 2025)

Research paper View study

+15–47%

Pesticide removal

Cowpea. Ozone microbubbles vs. conventional ozone bubbles (Li et al., 2023)

Research paper View study

4 log or more

Bacterial reduction

Tomato, 30-second wash with ozone microbubble water. Vs. water wash; reported values 4.11–4.83 log (Hou et al., 2022)

Research paper View study

Our own tests in produce and food washing are in progress. Published research is presented first.

  • Produce & food washing
Own cases
Validation in progress
Research cases
4

Results reported in published research

These are results from published research, not performance measured with Honour Tech products. We selected representative studies from peer-reviewed papers and public research reports and do not include corporate promotional material. Studies in which the effect was unclear are included as well. Last reviewed: October 2026.

Positive studies 3

  • 77.3%

    Pesticide removal from apples

    Bacteria and yeasts reduced by more than 3 log. Result obtained in combination with an antimicrobial.

    77.3% pesticide removal from apples with air micro/nanobubbles and an antimicrobial

    Bacteria and yeasts reduced by more than 3 log. Result obtained in combination with an antimicrobial.

    Paper details and results

    Decontamination of fresh apples using air micro-nanobubbles with epsilon-poly-lysine

    Decontamination of apples using air micro-nanobubbles and ε-polylysine

    Citation
    Lu et al. · 2025 · Food Innovation and Advances 4(4), 446–453
    Summary
    Apples inoculated with Listeria, spoilage yeast and a pesticide (trichlorfon) were washed with air micro-nanobubble water combined with an antimicrobial (ε-polylysine 250 mg/L) and compared with each treatment alone.
    Key findings
    The pesticide removal of 77.3% was significantly higher than with either treatment alone, and quality after 20 days of storage did not differ. This removal rate was obtained with nanobubbles and the antimicrobial combined; the contribution of each was not evaluated separately.
    Gas used
    Air
    View original (opens in a new window)
  • +15–47%

    Additional removal of 5 pesticides

    Cowpea, vs. conventional ozone bubbles. A microbubble study.

    Pesticide removal up an additional 15–47% with ozone microbubble washing

    Cowpea, vs. conventional ozone bubbles. A microbubble study.

    Paper details and results

    Accelerated removal of five pesticide residues in three vegetables with ozone microbubbles

    Accelerated removal of five pesticide residues in three vegetables with ozone microbubbles

    Citation
    Li et al. · 2023 · Food Chemistry 403, 134386
    Summary
    Celery, bok choy and cowpea sprayed with 5 pesticides in the field were washed with ozone microbubbles or conventional ozone bubbles, and removal rates were compared.
    Key findings
    Overall removal from cowpea was relatively low at 28–65%, which the authors attributed to the surface structure of the crop. This is a microbubble study, and statistical significance was not reported.
    Gas used
    Ozone
    View original (opens in a new window)
  • 4.11–4.83 log

    Bacterial reduction on tomatoes

    Vs. water wash. Ozone microbubble water

    Bacteria on tomatoes reduced by 4.11–4.83 log with ozone microbubble water washing

    Vs. water wash. Ozone microbubble water

    Paper details and results

    Antibacterial Efficacy and Physiochemical Effects of Ozone Microbubble Water on Tomato

    Antibacterial efficacy and physicochemical effects of ozone microbubble water on tomato

    Citation
    Hou et al. · 2022 · Sustainability 14(11), 6549
    Summary
    Tomatoes inoculated with 4 foodborne pathogens were washed for 30 seconds with ozone microbubble water (bubbles 3.84–11.99 μm) and compared with washing in water, microbubbles alone and ozone alone.
    Key findings
    Bacterial counts were 3.37–4.50 log lower than with ozone alone and 2.09–2.82 log lower than with microbubbles alone. Texture and aroma did not differ. Dissolved ozone in the ozone microbubble water (over 10 mg/L) was much higher than in the ozone-only water (about 2 mg/L), so the effect of the bubbles is mixed with that of ozone concentration.
    Gas used
    Ozone
    View original (opens in a new window)

Neutral studies 1

  • No significant difference found

    Oxygen nanobubbles alone

    Bacteria were significantly reduced only when combined with ultrasound.

    No bacterial reduction found with oxygen nanobubble washing alone

    Bacteria were significantly reduced only when combined with ultrasound.

    Paper details and results

    The Effect Ultrasound and Surfactants on Nanobubbles Efficacy against Listeria innocua and Escherichia coli O157:H7, in Cell Suspension and on Fresh Produce Surfaces

    Effect of ultrasound and surfactant on bacterial reduction by nanobubbles: bacterial suspensions and produce surfaces

    Citation
    Rafeeq, Ovissipour · 2021 · Foods 10(9), 2154
    Summary
    Two bacteria in suspension and on spinach leaves were treated with oxygen nanobubbles, ultrasound, both combined, and with added surfactant.
    Key findings
    In suspension, nanobubbles alone or ultrasound alone produced no significant reduction even after 15 minutes. Combined, they removed more than 2 log of Listeria and more than 4 log of E. coli from spinach. Adding surfactant did not significantly increase the effect.
    Gas used
    Oxygen
    View original (opens in a new window)

Frequently Asked Questions

Which produce has been studied?

We include studies on apples, tomatoes, three vegetables and spinach.

The studies address changes in bacterial counts and pesticide residues. Results vary with the type and surface condition of the produce, the contaminants and the washing conditions.

Which approaches had the largest effect?

The effect was largest when bubbles were combined with ozone, an antimicrobial or ultrasound.

One study reported that water with bubbles alone did not reduce bacteria. In the ozone studies, the effect of the bubbles is mixed with that of ozone concentration.

When will Honour Tech's test data be available?

Our own tests are in progress.

We will publish the results on this page once they are compiled. Tell us what you wash and how water is supplied, and we will propose a configuration suited to your site.

How were the studies selected?

We selected representative studies from peer-reviewed published papers.

We include 3 positive studies and 1 study with limited effect. These are not performance measurements of Honour Tech products, and most of the studies address microbubbles or micro-nanobubbles.

Tell us what you wash, how water is supplied and your hourly water use, and we will propose a configuration suited to your site.

Contact Us